کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167307 | 1092323 | 2011 | 8 صفحه PDF | دانلود رایگان |
Cedar pollens cause severe allergic disease throughout the world. We have previously characterized allergenic pollen glycoproteins from mountain cedar (Juniperus ashei) that bind to allergen-specific immunoglobulin E (IgE). In the present report, we investigated an alternative pathway of mast cell activation by mountain cedar pollen extract through IgE-independent mechanisms. We show that mountain cedar pollen directly induces mast cell serotonin and IL-4 release and enhances release induced by IgE cross-linking. Concomitant with mediator release, high levels of intracellular reactive oxygen species (ROS) were generated, and both ROS and serotonin release were inhibited by anti-oxidants. These findings suggest that alternative mechanisms exist whereby pollen exposure enhances allergic inflammatory mediator release through mechanisms that involve ROS. These mechanisms have the potential for enhancing the allergenic potency of pollens.
► We investigated an alternative pathway of mast cell activation by mountain cedar pollen through IgE-independent mechanisms.
► Pollen induces oxidative stress, degranulation and cytokine production in mast cells.
► Pollen enhances IgE-dependent degranulation and cytokine production in mast cells.
► Antioxidant treatment suppresses the pollen-induced activation of mast cells.
Journal: Cellular Immunology - Volume 271, Issue 2, 2011, Pages 488–495