کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167377 | 1092327 | 2012 | 5 صفحه PDF | دانلود رایگان |
G-protein coupled receptor kinase-5 (GRK5) is a recently described NFκB regulator in TLR4 signaling pathway. To determine whether the role of GRK5 is MyD88- or TRIF-dependent, we injected wild type and GRK5 knockout mice with Pam3CSK4 (MyD88-dependent TLR1/2 ligand) and Poly(I:C) (TRIF-dependent TLR3 ligand) and examined the in vivo systemic inflammatory response. Our results demonstrate that GRK5 regulates IL-12p40 and G-CSF via a mechanism that is common to both MyD88 and TRIF. However, GRK5 regulates IL-5 and MCP-1 in a MyD88-dependent but TNFα in a TRIF-dependent manner. Together, our results demonstrate multiple roles of GRK5 in TLR signaling.
► TLR-induced IL-12p40 and G-CSF are mediated by GRK5 via both MyD88- and TRIF-dependent pathways.
► TLR-induced increase in IL-5 and MCP-1 are regulated by GRK5 in a MyD88-dependent fashion.
► TLR-induced TNFα and RANTES are regulated by GRK5 in a TRIF-dependent manner.
Journal: Cellular Immunology - Volume 272, Issue 2, 2012, Pages 107–111