کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2168148 1092379 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Treatment of Brucella-susceptible mice with IL-12 increases primary and secondary immunity
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Treatment of Brucella-susceptible mice with IL-12 increases primary and secondary immunity
چکیده انگلیسی

Brucella spp. cause disease in humans and livestock and are potential biowarfare agents. Defining the protective immune response is necessary to design vaccines. This has largely been done with mice, brucella-susceptible BALB/c and resistant C57BL strains. Since interferon-γ is key to brucella resistance, contrary to expectations, we found that ex vivo splenocytes from naïve BALB/c mice produced IL-12 and interferon-γ in cultures with brucellae at levels comparable to those of splenocytes from the more resistant C57BL/10 mice. Moreover, both IL-12 and interferon-γ were produced in the first week following infection of BALB/c mice. However, by the third week of infection we found decreased IL-12Rβ2 expression by BABL/c splenocytes, corresponding to their inability to produce interferon-γ in Brucella recall responses at this time as reported previously. Administering recombinant IL-12 to these mice ameliorated the interferon-γ hiatus, resulted in a 1000-fold reduction in CFU during primary infection and increased survival following secondary challenge.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 243, Issue 1, September 2006, Pages 1–9
نویسندگان
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