|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2199592||1099601||2016||3 صفحه PDF||ندارد||دانلود رایگان|
• Different methods have been described to detect the EDNRBIle118Lys mutation, including AS-PCR and PIRA-PCR.
• These tests, which have been carried out by polyacrylamide gel electrophoresis (PAGE), are time-consuming techniques.
• In our study, we developed a new method based on mutagenically separated PCR (MS-PCR).
• Our method provides a fast and simple analysis of the EDNRB horse gene.
Lethal white foal syndrome (LWFS) is an autosomal recessive disease of neonatal foals characterized by a white hair coat and a functional intestinal obstruction. Traditional techniques for identifying the dinucleotide mutation (TC→AG) of the endothelin receptor B gene (EDNRB) associated with LWFS are time-consuming. We developed a new technique based on mutagenically separated polymerase chain reaction (MS-PCR) for simple detection of the EDNRB genotype in horses.
Journal: Molecular and Cellular Probes - Volume 30, Issue 3, June 2016, Pages 182–184