کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2199592 1099601 2016 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
New test for endothelin receptor type B (EDNRB) mutation genotyping in horses
ترجمه فارسی عنوان
آزمایش جدید برای ژنوتیپ جهش نوع B گیرنده اندوتلین (EDNRB) در اسب
کلمات کلیدی
اسب؛ LWFS؛ ژن EDNRB؛ واکنش زنجیره ای پلیمراز جدا شده از Mutagenically
Horse; LWFS; EDNRB gene; Mutagenically separated polymerase chain reaction
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


• Different methods have been described to detect the EDNRBIle118Lys mutation, including AS-PCR and PIRA-PCR.
• These tests, which have been carried out by polyacrylamide gel electrophoresis (PAGE), are time-consuming techniques.
• In our study, we developed a new method based on mutagenically separated PCR (MS-PCR).
• Our method provides a fast and simple analysis of the EDNRB horse gene.

Lethal white foal syndrome (LWFS) is an autosomal recessive disease of neonatal foals characterized by a white hair coat and a functional intestinal obstruction. Traditional techniques for identifying the dinucleotide mutation (TC→AG) of the endothelin receptor B gene (EDNRB) associated with LWFS are time-consuming. We developed a new technique based on mutagenically separated polymerase chain reaction (MS-PCR) for simple detection of the EDNRB genotype in horses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 30, Issue 3, June 2016, Pages 182–184
نویسندگان
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