|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2199593||1099601||2016||3 صفحه PDF||ندارد||دانلود رایگان|
• A novel, real-time fluorescence assay has been developed for the detection of Dam methyltransferase (MTase) activity.
• The detection limits for Dam MTase is 0.5 unit/mL.
• This method has been applied to detect Dam MTase inhibitor.
• Because no labeling with a fluorophore-quencher pair was required, the method is fairly simple and low cost.
A real-time assay for DNA methyltransferase (MTase) activity has been developed. A hemimethylated smart probe is used as the substrate for DNA MTase. Cleavage of the methylated product leads to separation of fluorophore from quencher, giving a proportional increase in fluorescence. The method permits real-time monitoring of DNA methylation process and makes it easy to characterize the activity of DNA MTase. It also has the potential to screen suitable inhibitor drugs for DNA MTase.
Journal: Molecular and Cellular Probes - Volume 30, Issue 3, June 2016, Pages 185–187