کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2199593 1099601 2016 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Real-time monitoring of DNA methyltransferase activity using a hemimethylated smart probe
ترجمه فارسی عنوان
نظارت بر زمان واقعی فعالیت متیل ترانسفراز DNA با استفاده از پروب هوشمند hemimethylated
کلمات کلیدی
نظارت بر زمان واقعی؛ متیل ترانسفراز DNA؛ پروب های هوشمند؛ Hemimethylated
Real-time monitoring; DNA methyltransferase; Smart probe; Hemimethylated
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


• A novel, real-time fluorescence assay has been developed for the detection of Dam methyltransferase (MTase) activity.
• The detection limits for Dam MTase is 0.5 unit/mL.
• This method has been applied to detect Dam MTase inhibitor.
• Because no labeling with a fluorophore-quencher pair was required, the method is fairly simple and low cost.

A real-time assay for DNA methyltransferase (MTase) activity has been developed. A hemimethylated smart probe is used as the substrate for DNA MTase. Cleavage of the methylated product leads to separation of fluorophore from quencher, giving a proportional increase in fluorescence. The method permits real-time monitoring of DNA methylation process and makes it easy to characterize the activity of DNA MTase. It also has the potential to screen suitable inhibitor drugs for DNA MTase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 30, Issue 3, June 2016, Pages 185–187
نویسندگان
, , , , , ,