Flow Cytometry Probes to Evaluate Stallion Spermatozoa

• Flow cytometry allows rapid evaluation of multiple parameters simultaneously.
• New flow cytometry protocols have been recently developed.
• A high increase in the use of flow cytometry is expected in stallion andrology.

The aim of this review is to present the current probes available that assess different compartments and functions of stallion spermatozoa, including assays to investigate the functionality of the membranes, nucleus, and mitochondria. New multiparametric protocols for the assessment of stallion sperm, recently developed in the laboratory of the authors, will also be presented. The potential clinical applicability of a diagnostic test based on flow cytometry will also be discussed.