کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2401728 1102369 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tuberculosis genes expressed during persistence and reactivation in the resistant rabbit model
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Tuberculosis genes expressed during persistence and reactivation in the resistant rabbit model
چکیده انگلیسی

SummaryAs previously published, after aerosol infection with Mycobacterium tuberculosis H37Rv, New Zealand white rabbits established infection with active bacillary replication, but later contained disease to a paucibacillary state through an effective adaptive response consistent with latency. Despite the heterogeneity among outbred rabbits, the resistant response was uniform. Immunosuppression resulted in reactivation with increased lung bacillary burden. Using this rabbit model, we isolated bacillary RNA from infected rabbit lungs and assessed transcriptional profiles of bacillary genes using RT-PCR to examine genes differentially regulated during active replication, persistence, steroid-induced reactivation, and post-steroid immune reconstitution. Genes involved in hypoxia response (fdxA), resuscitation promoting factors (rpfB), and DNA repair pathways (Rv2191) may be important in bacillary persistence. Further investigation into these gene pathways is warranted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tuberculosis - Volume 89, Issue 1, January 2009, Pages 17–21
نویسندگان
, , , , ,