کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2431561 1106763 2017 3 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
A multicolour flow cytometry identifying defined leukocyte subsets of rainbow trout (Oncorhynchus mykiss)
ترجمه فارسی عنوان
شناسایی جریان رنگارنگ cytometry زیر مجموعه های لکوسیت تعریف شده از قزل آلای رنگین کمان (Oncorhynchus mykiss)
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
چکیده انگلیسی


• Method for discrimination of leukocyte subpopulations from 10 μl of peripheral blood.
• Established approach does not require a density gradient centrifugation.
• All major leukocyte subpopulations are identified within one tube.
• Allows distinction between CD8+ and CD8− T cells (including putative CD4+ T cells).

The investigation of the cellular immune response in fish species has been for a long time hampered by absence of appropriate monoclonal antibodies (MAbs) recognising subset specific surface markers. Consequently, the majority of immunological studies still focus on the changes in total leukocyte numbers or describe gene pattern in lymphoid organs without any information about their cellular composition.Flow cytometric techniques are routinely used for the evaluation of the leukocyte composition in numerous vertebrate species and contributed significantly to the current knowledge of immune system. In rainbow trout is so far only a limited number of MAbs against characterised (IgM and IgT, CD8α) or unknown lineage markers on thrombocytes, myeloid cells or T cells available. By combination of several MAbs, we developed a rapid, simple, accurate and high throughput method for reliable discrimination of major leukocyte subpopulations from 10 μl of peripheral blood. Additionally, by a consecutive gating, this mixture enables the evaluation of the proportion between CD8α+ and CD8α− population and provides for the first time valuable information about the kinetic of CD4+ cells in rainbow trout. Furthermore, the combination of all antibodies within one sample reduced the hands-on time down to 90 min allowing fast and accurate estimation of cell kinetics in a high number of individuals.Thus presented findings enable the precise evaluation of the cellular components of immune system during both pathological and physiological responses and have therefore an immense potential for future applications in the development of vaccines and better understanding of fish immune system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 35, Issue 6, December 2013, Pages 2017–2019
نویسندگان
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