کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2466476 1555339 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Shiga toxin-producing Escherichia coli (STEC): Zoonotic risks associated with psittacine pet birds in home environments
ترجمه فارسی عنوان
اشرشیاکلی تولیدکننده سم شیگا (STEC): خطرات مشترک انسان و دام در ارتباط با پرندگان حیوان خانگی در محیط خانه
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


• E. coli isolated from psittacine birds in Brazil were eae+ and stx2+; this fact highlights the zoonotic risk of these animals.
• The E. coli strains isolated from psittacine birds were classified as non-O157 Shiga toxin producing Escherichia coli (STEC).
• Some STEC isolates were classified within the B2 phylogroup and were clonally grouped using AFLP.

Psittacidae are frequentely bred as pets worldwide, but little is known about the zoonotic risks of these animals. The objective of this study was to investigate the presence of Shiga toxin-producing Escherichia coli (STEC) in the feces of psittacine birds housed as pets. A total of 171 fecal samples (67 cockatiels, 59 budgerigars, and 45 agapornis) were cultured. Forty-two (E. coli) strains were identified, and the presence of the eae, stx1, and stx2 genes was determined using PCR. The antimicrobial resistance profiles of the STEC strains were determined using the disk diffusion method and phylogenetic analysis according to the new Clermont phylotyping method. Using these methods, 19.4% (8/42) of the STEC strains were determined to be positive for the eae and stx2 genes. The results revealed a STEC frequency of 4.6% in the birds (8/171), with a percentage of 8.47% in budgerigars (5/59), 4.47% in cockatiels (3/67), and 0% in agapornis (0/45). None of the STEC isolates belonged to the O157 serogroup. Most of the strains were classified as sensitive to the 18 antibiotics tested. None of the strains exhibited a multiresistance profile. In the phylogenetic analysis, two strains were classified as non-typeable, three were classified as B2, two were classified as F, and one was classified as Clade I. Seven of the eight STEC strains showed a clonal profile using AFLP. E. coli strains that are stx2+ plus eae+ are usually associated with severe human diseases such as hemorrhagic colitis and hemolytic-uremic syndrome. The STEC-positive results indicate the zoonotic risk of breeding psittacidae in home environments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 184, 29 February 2016, Pages 27–30
نویسندگان
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