کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2538023 1559631 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immunochromatographic strip assay for detection of bioactive Ganoderma triterpenoid, ganoderic acid A in Ganoderma lingzhi
ترجمه فارسی عنوان
تست نوار ايمونو كروماتوگرافی برای تشخيص زيست فعال گانودرما تریترپنوئيد، اسيد گندوريك اسيد A در گانودرما لينژي
کلمات کلیدی
طلای کلوئیدی؛ اسید Ganoderic؛ Ganoderma lingzhi؛ آزمایش ایمونو کروماتوگرافی؛ آنتی بادی مونوکلونال
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
چکیده انگلیسی

Ganoderic acid A (GAA) is one of the major Ganoderma triterpenes produced by medicinal mushroom belonging to the genus Ganoderma (Ganodermataceae). Due to its interesting pharmacological activities, Ganoderma species have been traditionally used in China for the treatment of various diseases. Herein, we developed a colloidal gold-based immunochromatographic strip assay (ICA) for the rapid detection of GAA using highly specific monoclonal antibody against GAA (MAb 12A) conjugated with gold nanoparticles. Using the developed ICA, the detection of GAA can be completed within 15 min after dipping the test strip into an analyte solution with the limit of detection (LOD) for GAA of ~ 500 ng/mL. In addition, this system makes it possible to perform a semi-quantitative analysis of GAA in Ganoderma lingzhi, where high reliability was evaluated by enzyme-linked immunosorbent assay (ELISA). The newly developed ICA can potentially be applied to the standardization of Ganoderma using GAA as an index because GAA is major triterpenoid present much in the mushroom.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fitoterapia - Volume 114, October 2016, Pages 51–55
نویسندگان
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