کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2580026 1561593 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Caffeine prevents LPS-induced inflammatory responses in RAW264.7 cells and zebrafish
ترجمه فارسی عنوان
کافئین مانع پاسخ التهابی ناشی از LPS در سلولهای RAW264.7 و ماهیان دریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


• Caffeine inhibits LPS-induced pro-inflammatory factor by regulating gene expression in RAW264.7 cells.
• Caffeine regulates NF-κB activation and phosphorylation of MAPKs.
• Caffeine suppresses LPS-induced NO production by using DCF-FM DA in zebrafish.

Caffeine is a white crystalline xanthine alkaloid found in the seeds of coffee plants and leaves of the tea bush. In this study, we evaluated whether caffeine exerts anti-inflammatory effects on lipopolysaccharide (LPS)-induced inflammation both in vitro and in vivo. RAW264.7 cells were treated with various concentrations of caffeine in the presence or absence of LPS. Caffeine decreased the LPS-induced inflammatory mediator, nitric oxide (NO). Caffeine treatment also reduced the expression of pro-inflammatory genes, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-3, IL-6 and IL-12, and decreased both IL-6 secretion and phosphorylated p38MAPK expression in LPS-treated RAW264.7 cells. Caffeine inhibited nuclear translocation of nuclear factor κB (NF-κB) via IκBα phosphorylation. In addition, caffeine inhibited LPS-induced NO production in zebrafish. These results suggest that caffeine may suppress LPS-induced inflammatory responses in RAW264.7 cells by regulating NF-κB activation and MAPK phosphorylation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 248, 25 March 2016, Pages 1–7
نویسندگان
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