Enzyme-containing silica inverse opals prepared by using water-soluble colloidal crystal templates: Characterization and application

• Enzyme-containing silica inverse opals were prepared using water-soluble PAM CCTs.
• Enzyme-containing silica inverse opals showed improved stabilities than free enzymes.
• Enzyme-containing silica inverse opals were used for detections of glucose and OPs.

Enzyme-containing silica inverse opals are prepared for the first time by using water-soluble polyacrylamide (PAM) colloidal crystals as templates. Glucose oxidase-containing silica inverse opals (GOD@SIOs) are firstly fabricated and characterized by SEM, TEM, CLSM, and N2 adsorption-desorption. Compared with free GOD, the GOD@SIOs are more stable against extreme pH, heat, and chemical denaturants. GOD@SIOs can be used in fast and sensitive visual detection of glucose. In order to verify that this method is suitable for various enzymes, organophosphorus hydrolase (OPH)-containing silica inverse opals are also prepared and successfully applied as sensors for visual and spectrometric detection of organophosphorus based compounds. These enzyme-containing silica inverse opals offer a novel approach to in situ immobilize biomolecules.

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