Residual antibiofilm effects of various concentrations of double antibiotic paste used during regenerative endodontics after different application times

• We investigated the residual antibacterial effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks.
• Dentin pretreatment with 5 mg/mL of DAP or higher demonstrated significant and substantial residual antibacterial effects regardless of the treatment time.
• Dentin pretreatment for 4 weeks with 5 mg/mL of DAP or higher demonstrated significantly higher residual antibacterial effects in comparison to a 1 week pretreatment with similar concentrations.
• Dentin pretreatment with calcium hydroxide did not demonstrate any substantial residual antibacterial effect regardless of the treatment time.

ObjectiveWe investigated the residual antibiofilm effects of different concentrations of double antibiotic paste (DAP) applied on radicular dentin for 1 or 4 weeks.DesignDentin samples were prepared (n = 120), sterilized and pretreated for 1 or 4 weeks with the clinically used concentration of DAP (500 mg/mL), low concentrations of DAP (1, 5 or 50 mg/mL) loaded into a methylcellulose system, calcium hydroxide (Ca(OH)2), or placebo paste. After the assigned treatment time, treatment pastes were rinsed off and the samples were kept independently in phosphate buffered saline for 3 weeks. Pretreated dentin samples were then inoculated with Enterococcus faecalis and bacterial biofilms were allowed to grow for an additional 3 weeks. Biofilms were then retrieved from dentin using biofilm disruption assays, diluted, spiral plated, and quantified. Fisher’s Exact and Wilcoxon rank sum tests were used for statistical comparisons (α=0.05).ResultsDentin pretreatment for 4 weeks with 5, 50 or 500 mg/mL of DAP demonstrated significantly higher residual antibiofilm effects and complete eradication of E. faecalis biofilms in comparison to a 1 week pretreatment with similar concentrations. However, dentin pretreated with 1 mg/mL of DAP or Ca(OH)2 did not provide a substantial residual antibiofilm effect regardless of the application time.ConclusionsDentin pretreatment with 5 mg/mL of DAP or higher for 4 weeks induced significantly higher residual antibiofilm effects in comparison to a 1 week pretreatment with the same concentrations.