کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3120618 1583285 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of risedronate on osteoblastic cell cultures
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Effects of risedronate on osteoblastic cell cultures
چکیده انگلیسی


• The effects of bisphosphonates on osteoblast are controversial.
• Risedronate stimulates Type I Collagen synthesis by osteoblastic cells.
• ALP activity in osteoblastic cells was enhanced by risedronate.
• Risedronate promoted extracellular matrix mineralization.

ObjectiveBisphosphonates (BPs) have been widely used in the treatment of bone disorders due to their ability to modulate bone turnover. The biological mechanisms through BFs exert their effects on osteoclasts are well established. However, the role of BFs on the osteoblasts is controversial. The present study aimed to evaluate the effects of risedronate on osteoblastic cells.DesignMC3TE-E1 cells were exposed to risedronate at 0, 10−8, 10−6, 10−4, and 10−3 M. The following parameters were assayed: (1) cell proliferation by hemocytometer counting after 24, 48 and 72 h, (2) cell viability by MTT assay after 24, 48 and 72 h, (3) Type I Collagen quantification by ELISA after 24, 48 and 72 h, (3) alkaline phosphatase activity after 7 and 10 days and (4) matrix mineralization after 14 days.ResultsAfter 24 h, risedronate did not affect both cell proliferation and viability (p > 0.05). However, after 48 and 72 h, a decrease in cell proliferation and viability was detected in osteoblastic cultures exposed to risedronate at 10−4 and 10−3 M (p < 0.05). After 48 and 72 h, Type I Collagen synthesis was stimulated by risedronate at 10−4 M (p < 0.05). High levels of ALP activity were detected in cultures exposed to risedronate at 10−4 M after 7 and 10 days (p < 0.05). After 14 day, high calcium content was observed in cultures exposed to risedronate at 10−4 M (p > 0.05).ConclusionThese results indicated that risedronate can promote osteoblast differentiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 68, August 2016, Pages 43–47
نویسندگان
, , , , , , , ,