Synthesize of Titanium dioxide nanoparticles using Moringa oleifera leaves and evaluation of wound healing activity

Titanium dioxide nanoparticles (TiNPs) were emerged as wound healing enhancer as well as an antibacterial agent against Gram-positive and Gram-negative bacteria due to its enhanced mechanical properties. Moreover, the green synthesized Titanium dioxide exhibited significant wound healing activity in Albino rats which was confirmed by measuring wound closure. Titanium dioxide nanoparticles were effectively synthesized using aqueous leaf extract of Moringa oleifera under pH and temperature dependent condition. 5 mM Titanium dioxide solution worked as a primary source for the synthesize of Titanium dioxide nanoparticles. The green synthesized TiNPs excitation was confirmed using UV–Vis spectrophotometer at 428 nm. The high-resolution Scanning Electron Microscope (SEM) results showed the spherical shape of TiNPs with a mean particle size around 100 nm. Furthermore, the wound healing activity of nano-sized particles (Control Group) when compared with micro-sized (Positive Control Group) was examined through the excision wound model by measuring wound closure and the control group revealed significant wound healing activity in Albino rats.