کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3278492 1589772 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microbiological evaluation of anatomical organs submitted to glycerinization and freeze-drying techniques
ترجمه فارسی عنوان
ارزیابی میکروبیولوژیکی از ارگان های تشریحی ارائه شده به glycerinization و تکنیک های خشک کردن فریزی
کلمات کلیدی
اندام حفاظت؛ تکنیک های تشریحی. میکروبیولوژی؛ رشد میکروبی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
چکیده انگلیسی


• Alternatives conservation techniques are useful nowadays.
• Formaldehyde substitution for techniques of glicerinization and freeze-drying.
• Microbiological analyzes are essential to detect possible deteriorative microorganisms.
• Spread plate of total moulds, mesophiles, psychrophilic and Pseudomonas sp.
• Both alternatives techniques resulted in zero or in very low microbial quantity.

Alternatives conservation techniques are being requested with the proposal of formaldehyde substitution. Formaldehyde results in excessive anatomical specimens' weight and it can cause serious health problems to the manipulator, such as cancer. However, it provides an efficient germicide and fungicide action depending on concentration. The substitute techniques are glicerinization and freeze-drying which have advantages such as non-production of smells, lightness of the organs and dispenses the use of fixatives in conservation. As well as both intrinsic and extrinsic factors interfere in microbial growth, microbiological analyzes are essential to detect possible deteriorative microorganisms in organs and concluding effectively the technique used. Formalinized, glycerinated and freeze-drying organs were collected in three different times which were intercalated by two months, except formalinization that had one evaluation. The procedure required the use of sterilized swabs wetted in peptone water and molds measuring 5,0 cm x 10,0 cm positioned on two different piece's local resulting in 100 cm2 of area, to spread plate of total moulds, mesophiles (except in freeze-drying), psychrophilic (only in freeze-drying) and Pseudomonas sp (except in formalinization). All the plates were counted and compared between each technique's evaluations by variance analyzes. Both alternatives techniques resulted in zero or in very low microbial quantity to cause health problems as well as it preserve pieces morphology. All values of all analyzes resulted below 1/ml, showing that glicerinization and freeze-drying techniques are so as efficient as formaldehyde.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research in Anatomy - Volumes 3–4, June–September 2016, Pages 1–4
نویسندگان
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