کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3373136 | 1219283 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biofilm formation by persistent and non-persistent isolates of Staphylococcus epidermidis from a neonatal intensive care unit
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
Correlation of biofilm formation and presence of the icaADBC genes was studied in 161 clinical isolates of S. epidermidis from persistent and non-persistent neonatal infections. Biofilm formation was compared in trypticase soy broth with or without added glucose (0.5-9.23%), and total parenteral nutrition (TPN) containing 9.23% glucose. Detection of icaADBC genes was carried out using polymerase chain reaction and ica-specific primers. Quantitative biofilm formation for all isolates was highest in the presence of 1% glucose, followed by trypticase soy broth and TPN. There was no significant difference between the amount of biofilm formed by persistent and non-persistent isolates under different test conditions. In contrast, 70% of the persistent isolates produced biofilm in TPN compared to the 56.3% of the non-persistent group. Neither the persistent bacterial phenotype nor presence of the icaADBC operon was correlated with biofilm formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hospital Infection - Volume 71, Issue 2, February 2009, Pages 112-116
Journal: Journal of Hospital Infection - Volume 71, Issue 2, February 2009, Pages 112-116
نویسندگان
F. Eftekhar, D.P. Speert,