کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3393731 1592763 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Elucidating the influence of praziquantel nanosuspensions on the in vivo metabolism of Taenia crassiceps cysticerci
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Elucidating the influence of praziquantel nanosuspensions on the in vivo metabolism of Taenia crassiceps cysticerci
چکیده انگلیسی


• The effect of the nanosuspensions on the energetic metabolism of cysticerci was proved.
• PZQ nanosuspensions increased the glycolysis in T. crassiceps cysticerci.
• Remarkable metabolic alterations produced by the stabilizers of the nanosuspensions were observed.
• Praziquantel nanosuspensions were succesfully prepared by nanoprecipitation process.

The aim of this work was to develop nanosuspensions of praziquantel (PZQ) and to evaluate their influence on the energetic metabolism of cysticerci inoculated in BALB/c mice. We analyzed metabolic alterations of glycolytic pathways and the tricarboxylic acid cycle in the parasite. The nanosuspensions were prepared by precipitation and polyvinyl alcohol (PVA), poloxamer 188 (P188) and poloxamer 407 (P407) were used as stabilizers. Nanosuspension prepared with PVA had a particle size of 100 nm, while P188- and P407-based nanosuspensions had particle sizes of 74 nm and 285 nm, respectively. The zeta potential was −8.1, −8.6, and −13.2 for the formulations stabilized with PVA, P188 and P407, respectively. Treatments of T. crassiceps cysticerci-infected mice resulted in an increase in glycolysis organic acids, and enhanced the partial reversion of the tricarboxylic acid cycle, the urea cycle and the production of ketonic bodies in the parasites when compared to the groups treated with conventional PZQ. These data suggest that PZQ nanosuspensions greatly modified the energetic metabolism of cysticerci in vivo. Moreover, the remarkable metabolic alterations produced by the stabilizers indicate that further studies on nanoformulations are required to find potentially suitable nanomedicines.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 161, September 2016, Pages 100–105
نویسندگان
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