کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3395091 1592831 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In-vitro activity of taurolidine on single species and a multispecies population associated with periodontitis
ترجمه فارسی عنوان
فعالیت آنزیم تروئیدولین بر روی گونه های تک و چند گونه ای همراه با پریودنتیت
کلمات کلیدی
تائورولیدین، مینوسیکلین، پاتوژنهای پریودنتال، بیوفیلم
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
چکیده انگلیسی


• We compared taurolidine with minocycline against periodontopathogenic bacteria.
• Antimicrobials were less active against a 12-species mixture.
• Antimicrobials reduced but did not eliminate viable bacteria within biofilms.
• Taurolidine might be an alternative in periodontitis treatment.

The antimicrobial activity of taurolidine was compared with minocycline against microbial species associated with periodontitis (four single strains and a 12-species mixture). Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs), killing as well as activities on established and forming single-species biofilms and a 12-species biofilm were determined. The MICs of taurolidine against single species were always 0.31 mg/ml, the MBCs were 0.64 mg/ml. The used mixed microbiota was less sensitive to taurolidine, MIC and the MBC was 2.5 mg/ml. The strains and the mixture were completely killed by 2.5 mg/ml taurolidine, whereas 256 μg/ml minocycline reduced the bacterial counts of the mixture by 5 log10 colony forming units (cfu). Coating the surface with 10 mg/ml taurolidine or 256 μg/ml minocycline prevented completely biofilm formation of Porphyromonas gingivalis ATCC 33277 but not of Aggregatibacter actinomycetemcomitans Y4 and the mixture. On 4.5 d old biofilms, taurolidine acted concentration dependent with a reduction by 5 log10 cfu (P. gingivalis ATCC 33277) and 7 log10 cfu (A. actinomycetemcomitans Y4) when applying 10 mg/ml. Minocycline decreased the cfu counts by 1–2 log10 cfu independent of the used concentration. The reduction of the cfu counts in the 4.5 d old multi-species biofilms was about 3 log10 cfu after application of any minocycline concentration and after using 10 mg/ml taurolidine. Taurolidine is active against species associated with periodontitis, even within biofilms. Nevertheless a complete elimination of complex biofilms by taurolidine seems to be impossible and underlines the importance of a mechanical removal of biofilms prior to application of taurolidine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Anaerobe - Volume 32, April 2015, Pages 18–23
نویسندگان
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