Adoptive transfer of FTY720-treated immature bone marrow-derived dendritic cells (BMDCs) significantly reduced the spontaneous resorption rate in the CBA/J × DBA/2 mouse model

ObjectiveTo investigate the effect of FTY720-treated immature bone marrow-derived dendritic cells (BMDCs) on the embryo resorption rate in the CBA/J× DBA/2 abortion mouse model.MethodsThe dendritic cells (DCs) were derived from bone marrow of DBA/2 mice, and then co-cultured with FTY720. The abortion mouse models were established by mating female CBA/J mice with DBA/2 mice. Via the CBA/J×DBA/2 abortion mouse model, six groups were established, group A: normal pregnancy model; group B: abortion mouse model with no treatment; group C: abortion mouse model injected with DC culture medium (DCCM); group D: abortion mouse model injected with DC; group E: abortion mouse model injected with FTY720; group F: abortion model mouse injected with FTY720-DC. The differences were compared in the embryo resorption rates of the CBA/J ×DBA/2 abortion mouse model treated with FTY720-DC or different controls observed on gestation day 12 to 14, and then the microenvironment in murine pregnancy was investigated.ResultsThe embryo resorption rate was statistically significantly decreased in group D and group E when they compared with group B and group C (P<0.05, respectively). Furthermore, the embryo resorption rate in group F showed a statistically significant decrease when compared with the other groups except group A (P<0.01). These results suggest that FTY720-DCs possess a notable advantage over DCs or FTY720 in reducing the embryo resorption rate of the abortion mouse model. The percentage of Th17 (IL-17+CD4+T cells) in peripheral blood mononuclear cell (PBMC) in the abortion mouse model was 4.35%±0.34% before treated with FTY720-DC, and was 1.34%±0.28% after treated with FTY720-DC (P<0.01). The percentage of Tregs (CD4+CD25+Foxp3+T cells) in PBMC was significantly increased in group F (8.35%±1.80%) as compared with group B (2.68%±0.65%)(P<0.01).ConclusionAdoptive transfer of FTY720-DC can statistically significantly reduce the embryo resorption rate in the CBA/J×DBA/2 abortion mouse model. The lower embryo resorption rate in the FTY720-DC treated abortion mouse model may be caused by the imbalance of Treg/Th17.