Photosynthetic response to different light intensities, water status and leaf age of two Berberis species (Berberidaceae) of Patagonian steppe, Argentina

Net photosynthetic rate (A) and stomatal conductance (gs) from leaves of Berberis buxifolia and Berberis heterophylla saplings were measured under different conditions of radiation and water availability and for leaves of different ages. Comparative studies of basic physiological performance would give insight how these shrubs survive and grow under this austral extreme ecosystem. B. buxifolia had higher A values than B. heterophylla for all light intensities measured. Values of gs for both Berberis species also declined as light intensity decreased. There was a negative relationship between A and water stress expressed as pre-dawn leaf water potential (ψlp) where the reduction in gs was the main factor that reduced A in both species. Also, A and gs of Berberis plants increased rapidly during the first days after leaf unfolding, reached a maximum value when the leaf was completely expanded, and then declined. These results could be used to estimate the responses of saplings to environmental conditions under the dynamic of natural stands in this austral extreme ecosystem and for restoration plans.

► We measured net photosynthetic rate (A) and stomatal conductance (gs) of Berberis.
► B. buxifolia had higher A values than B. heterophylla for all light intensities.
► There was a negative relationship between A and water stress.
► A and gs of Berberis increased rapidly during the first days after leaf unfolding.
► These results help to estimate the responses of saplings to environmental conditions.