کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4406363 1618681 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential effects of anti-cancer and anti-hepatitis drugs on liver cystatin
ترجمه فارسی عنوان
اثرات افتراق داروهای ضد سرطان و ضد هپاتیت بر سیستین کبدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بوم شناسی
چکیده انگلیسی

The drug–protein interaction has been the subject of increasing interest over the decades. In the present communication, the interaction of liver cystatin with anti-cancer (adriamycin) and anti-hepatitis (adevofir dipivoxil) drugs was studied by thiol-protease inhibitory assay, UV absorption, fluorescence spectroscopy and circular dichroism (CD). A static type of quenching was observed between the protein and the drug molecules. Binding constant (Ka) of adriamycin to liver cystatin (LC) was found to be 1.08 × 106 M−1. Moreover, binding site number was found to be 2. Importantly, cystatin loses its activity in the presence of adriamycin. However, intrinsic fluorescence studies in the presence of adevofir dipivoxil showed enhancement in the fluorescence intensity suggesting that binding of adevofir to LC caused unfolding of the protein. The unfolding of the test protein was also accompanied by significant loss of inhibitory activity. CD spectroscopy result showed, both adriamycin and adevofir dipivoxil caused perturbation in the secondary structure of liver cystatin. The possible implications of these results will help in combating drug induced off target effects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Saudi Journal of Biological Sciences - Volume 22, Issue 1, January 2015, Pages 69–74
نویسندگان
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