کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4407881 1618822 2016 9 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Bisphenol A disrupts microtubules and induces multipolar spindles in dividing root tip cells of the gymnosperm Abies cephalonica
ترجمه فارسی عنوان
بیسفنول A میکروتوبول ها را مختل می کند و اسپیندل های چند قطبی را در سلول های نابالغ ریشه گیاه شناسی Abies cephalonica
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Bisphenol A disrupts microtubules and induces multipolar spindles in dividing root tip cells of the gymnosperm Abies cephalonica
چکیده انگلیسی


• BPA disrupted microtubules in all mitotic stages of Abies cephalonica.
• Chromosomal aberrations were underlain by microtubule derangement under BPA stress.
• Endoplasmic reticulum malformations reflected those of microtubules.
• Microtubule recovery after oryzalin treatment was more effective in BPA than in water.
• BPA-induced multipolar spindles are attributed to the centrosomal properties of gymnosperms.

The effects of bisphenol A (BPA), an endocrine chemical disruptor extensively used in the plastic and epoxy resin industry, on dividing root tip cells of the gymnosperm Abies cephalonica Loudon were investigated by confocal laser scanning microscopy after tubulin and endoplasmic reticulum immunolocalization and DNA staining. Microtubule arrays of all mitotic stages were disrupted within a few hours of treatment: preprophase bands exhibited asymmetric width; prometaphase, metaphase and anaphase spindles appeared sharply pointed, sigmoid or multipolar; phragmoplast microtubules were elongated and occasionally bended toward the daughter nuclei. Depending on the mitotic stage, the chromosomes appeared condensed at prophase, as a compact mass at metaphase and anaphase, unsegregated or bridged at telophase. Endoplasmic reticulum patterns were also affected, reflecting those of the respective microtubule arrays. Recovery of the microtubules after oryzalin treatment was more effective in a BPA solution than in water. It is concluded that the plant mitotic apparatus microtubules are very sensitive to BPA, the effect of which depends on the specific cell cycle stage. The formation of multipolar spindles is reminiscent of animal cells and is ascribed to the induction of multiple microtubule nucleation sites, deriving from the centrosomal properties of gymnosperms.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 149, April 2016, Pages 202–210
نویسندگان
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