کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4408184 | 1618832 | 2015 | 8 صفحه PDF | دانلود رایگان |
• BaP exposure resulted in altered expressions of SKN-1 and GCS-1 in C. elegans.
• BaP exposure altered the expressions of microRNA in C. elegans.
• The response of GCS-1 to BaP might be independent of the regulation of SKN-1.
• The microRNAs might be involved in the regulations of SKN-1 and GCS-1 expression.
In the present study oxidative stress induced by Benzo-α-pyrene (BaP) exposure and the potential involvements of microRNA were investigated. The Caenorhabditis elegans (C. elegans) was applied as model organism. The C. elegans at L1-stage were randomly divided into 4 groups and exposed to 0, 0.2, 2.0, and 20 μM BaP for 30 h. Expressions of SKiNhead-1 (SKN-1), gamma-glutamine cysteine synthase heavy chain (GCS-1), and their potential regulatory factors in insulin/IGF-1/FOXO signaling pathway and the p38 MAPK pathway were analyzed. The expressions of potentially involved microRNAs were investigated as well. Results demonstrated that expressions of SKN-1 and GCS-1 were altered significantly following BaP exposure (P < 0.05). Meanwhile, expressions of multiple related factors were changed after BaP treatments. The altered factors include AKT-1, DAF-16, glutathione synthetase (GSS-1), glutathione S-transferase-24 (GST-24), mitogen-activated protein kinase kinase-4 (MKK-4), multidrug resistance-associated protein-1 (MRP-1), and pyruvate dehydrogenase kinase-2 (PDHK-2) (P < 0.05). In addition, results showed that exposure to BaP led to altered expressions of microRNA. Out of the 28 tested microRNAs, expressions of miR-1, miR-355, miR-50, miR-51, miR-58, miR-796, miR-797, and miR-84 were modified. Findings of the present study include that BaP exposure caused oxidative stress in C. elegans. The expressional response of GCS-1 to BaP exposure might be independent of the regulation of SKN-1 in C. elegans. The microRNAs might be involved in the regulations of SKN-1 and GCS-1 expression following BaP exposure in C. elegans.
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Journal: Chemosphere - Volume 139, November 2015, Pages 496–503