کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4419366 1618943 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of an epsilon-class glutathione S-transferase involved in tolerance in the silkworm larvae after long term exposure to insecticides
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Characterization of an epsilon-class glutathione S-transferase involved in tolerance in the silkworm larvae after long term exposure to insecticides
چکیده انگلیسی


• Silkworms were selected with phoxim and fenpropathrin for several generations.
• BmGSTe2 was highly expressed in the midguts of insecticide-selected strains.
• The elevated peroxidase activity of BmGSTE2 was involved in insecticide tolerance.

This study assessed the effect of the pesticides on activity and expression of glutathione S-transferases (GSTs) in the midgut of the silkworm after intense selections by phoxim and fenpropathrin for five and four generations, respectively. GSTs activity towards cumene hydroperoxide (CHP), a substrate of GSH-dependent peroxidase, was significantly increased in the crude homogenate of midguts after long term exposure to the insecticides. An epsilon-class GST gene (BmGSTe2) was identified and showed elevated expression in the midguts of phoxim- and fenpropathrin-selected strains. Expression of BmGSTe2 was only detected in the midgut at transcriptional and translational levels. The recombinant BmGSTE2 possessed peroxidase activity and significantly inhibited by fenpropathrin, phoxim and chlorpyrifos in vitro. These results indicated that BmGSTE2 might be one of the enzymes involved in enhancing larval tolerance to the insecticides used. Furthermore, GST activity and expression level of BmGSTe2 might be used as biomarkers of organophosphorus and pyrethroid insecticide exposures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 120, October 2015, Pages 20–26
نویسندگان
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