کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4506285 1624343 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Season-long expression of Cry1Ac and Cry2Ab proteins in Bollgard II cotton in Australia
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Season-long expression of Cry1Ac and Cry2Ab proteins in Bollgard II cotton in Australia
چکیده انگلیسی

Bollgard II cotton has been grown commercially in Australia since 2003 for control of the primary target species Helicoverpa armigera (Hübner) and Helicoverpa punctigera (Wallengren) Larvae of both species have been reported to survive at low frequencies on Bollgard II with larvae >8 mm recorded in between 7 and 18% of the area planted to Bollgard II cotton between 2005/06 and 2007/08. F1 and F2 tests have shown that this is not due to the presence of resistance genes in the surviving larvae. To understand if fluctuations in the expression of the Cry proteins in Bollgard II allow some larvae to survive, plant tissue samples were taken from five Bollgard II cultivars throughout the growing season within fields and from different farms within a production region between 2007 and 2010. The data indicate that the expression of both Cry proteins is similar to the known resistance-monitoring diagnostic concentrations and relatively uniform between fields within a farm and between farms within a region, with less than one-third of the tests at this level of variation being significant. However, there were intra-seasonal changes in expression of both Cry proteins and differences in expression between plant structures and between cultivars for both Cry proteins. Further work is needed to establish if this variation in Cry protein content in Bollgard II cotton affects the control of Helicoverpa spp. in the field or whether plant-physiological and pest-behavioural factors underlie the occasional occurrence of Helicoverpa larval survival on Bollgard II cotton.


► Low frequency survival of Helicoverpa larvae sometimes occurs on Bollgard II cotton.
► We quantified the Cry1Ac and Cry2Ab endotoxin contents of Bollgard over 3 seasons.
► Both varied more with cultivar, plant part and time than within fields and regions.
► The Cry contents were similar to or higher than known diagnostic concentrations.
► Occasional larval survival on Bollgard appears due to behavioural and other factors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Crop Protection - Volume 44, February 2013, Pages 50–58
نویسندگان
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