کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4570107 | 1331372 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of a useful mutant by chronic irradiation in sweetpotato
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sweetpotato cultivar Kokei No. 14 was planted in a 60Co gamma field. Shoot apices from the plants irradiated with different doses were cultured on MS medium supplemented with 2.0 mg/l 2,4-D for the induction of embryogenic calluses and the formation of somatic embryos. Embryogenic calluses with somatic embryos were transferred to MS medium supplemented with 1.0 mg/l ABA to induce the germination of somatic embryos. Plantlets from somatic embryos developed into whole plants on the basal MS medium. The regenerated plants were transplanted in a field and from them one mutant with alteration of root flesh color and yield was obtained. The root flesh color had changed from light-yellow in the wild-type plant into orange in the mutant. The yield of storage roots increased significantly from 218.8 ± 60 g (FW) in the original plant to 646.9 ± 150 g (FW) in the mutant per plant. Carotenoids content of the storage roots in the mutant had significant difference compared to the wild-type at the 1% level. Peroxidase isozymes and RAPD polymorphism were also analyzed between the mutant and the wild-type plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 111, Issue 2, 4 January 2007, Pages 173-178
Journal: Scientia Horticulturae - Volume 111, Issue 2, 4 January 2007, Pages 173-178
نویسندگان
Yuping Wang, Fei Wang, Hong Zhai, Qingchang Liu,