کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4752085 1415993 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regular articleMgO-Fe3O4 linked cellulase enzyme complex improves the hydrolysis of cellulose from Chlorella sp. CYB2
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Regular articleMgO-Fe3O4 linked cellulase enzyme complex improves the hydrolysis of cellulose from Chlorella sp. CYB2
چکیده انگلیسی


- Xylan aldehyde acts as a linking molecule for nanoparticle and enzymes.
- MgO-Fe3O4 improved the recovery, hydrolysability and residual activity.
- Linking of enzyme to nanoparticle affects the nature of α-helix and ß-sheet.
- Maximum glucose yield of 91% was observed from Chlorella sp. CYB2.

In the present study, a novel catalyst was prepared by combining cellulase enzyme complex with metal oxides as a substrate activating and enzyme stabilizing component. The cellulose content obtained from Chlorella sp. CYB2 was treated with magnetic metal oxide linked enzyme complex through xylan aldehyde linking molecule. In order to improve the hydrolysis process, MgO was blended with Fe3O4 and the resulting complex had significant improvement in immobilization yield, activity recovery and hydrolysis of cellulose. Addition of xylan aldehyde as a linking molecule enhanced the enzyme binding onto metal nanoparticle. The results on hydrolysis showed the reduction in crystallinity of cellulose corresponding to the increase in enzymatic digestibility of cellulose. Under optimized conditions, the glucose yield obtained was 91% of theoretical maximum. The enhancement in hydrolysis is correlated with the degradation of large molecule into well accessible substrate assisted by the action of metal oxides.

85Synthesis of xylan aldehyde for linking MgO-Fe3O4 and cellulase to hydrolyse cellulose into glucose.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 122, 15 June 2017, Pages 22-30
نویسندگان
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