|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|4765728||1362360||2018||6 صفحه PDF||ندارد||دانلود کنید|
â¢A highly selective fluorescent probe based on fluorescein aldehyde is developed for HOCl detection.â¢The probe exhibited a perfect mitochondria targetable ability.â¢The probe can be used to detect mitochondrial HOCl and monitor the MPO-catalyzed HOCl generation in living cells.
In the present study, a mitochondria-targeted fluorescent probe FL based on fluorescein derivative for hypochlorite sensing was designed and synthesized. The probe FL showed highly selective to HOCl among various reactive oxygen/nitrogen species. With the increasing amount of HOCl from 0 to 50Â Î¼M, a linear correlation between the fluorescence intensity of FL at 530Â nm and the concentration of HOCl was found. The detection limit is calculated to be 0.068Â Î¼M. Furthermore, the probe demonstrated a perfect mitochondria targetable ability and it was successfully applied to image mitochondrial HOCl in living HeLa cells. The success of subcellular imaging indicated that the mitochondria-targetable probe FL might be further used to investigate biological functions and pathological roles of HOCl at organelle levels.
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Journal: Dyes and Pigments - Volume 148, January 2018, Pages 353-358