کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4914585 1362626 2018 10 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
A novel supercritical CO2-based decellularization method for maintaining scaffold hydration and mechanical properties
کلمات کلیدی
Tissue engineering; Decellularization; Supercritical CO2; Aorta; Dehydration;
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A novel supercritical CO2-based decellularization method for maintaining scaffold hydration and mechanical properties
چکیده انگلیسی

•Supercritical CO2 decellularization may be faster and gentler than other methods.•ScCO2 alone was unable to decellularize porcine aorta, even with ethanol added.•A hybrid detergent/scCO2 treatment fully and quickly decellularized porcine aorta.•Presaturating scCO2 with water prevented tissue dehydration during treatment.

Decellularized tissues are commonly utilized as tissue engineering scaffolds. Decellularization by extended exposure to aqueous detergents can damage the microstructure or deposit cytotoxic residue. Supercritical carbon dioxide (scCO2) has been proposed for decellularization, but reportedly causes dehydration and scaffold embrittlement.Presented herein is a novel decellularization method that preserves matrix hydration state and mechanical properties. Over 97% of the water in porcine aorta is maintained by presaturating scCO2 with water; however, complete decellularization was not attained by any process utilizing only scCO2. Instead, a novel hybrid method is presented that combines a brief (48 h) exposure of tissue to aqueous detergent, followed by washing with scCO2 (1 h). The hybrid method fully decellularized the tissue, as confirmed by histology and DNA quantification (<0.04 μg DNA/mg tissue). This hybrid treatment was faster than the standard method (2 days compared to 4–7 days), while preserving tissue structure and mechanical properties.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Supercritical Fluids - Volume 131, January 2018, Pages 72-81
نویسندگان
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