|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|4914586||1362626||2018||6 صفحه PDF||ندارد||دانلود رایگان|
â¢Flavonoids have been extracted using Supercritical fluid extraction method.â¢Flavonoids contents is higher in supercritical extracts than the conventional.â¢The response surface methodology allowed prediction of the optimum conditions.â¢Supercritical extracts presented higher antioxidant activity.
Flavonoids were extracted from Odontonema strictum leaves by supercritical carbon dioxide, with ethanol. The effect of three independents variables (time, pressure and temperature) on the Total Flavonoid Content (TFC) were optimized by factorial investigation. The TFC and flavonoid recovery varies respectively from 99.33 to 247.78Â mg/g of dried extract and 10.68â18.92Â mg/g of dried leaves powder, while the conventional solvent extraction (CSE) yielded 36.45Â mg/g and 3.13Â mg/g. Analysis of the factorial identified the optimal conditions with an extraction time of 270Â min and a pressure of 200Â bar, the temperature had no effect within the tested ranges. The predicted TFC was 203.11Â mg/g under optimal conditions and experimentally 230.48Â mg/g. The antioxidant activity with 2,2-Diphenyl-1-picrylhydrazyl method of the extracts under optimum conditions was 49.21% while the CSE extract was 37.05%. The TLC analysis of the supercritical fluid extracts from the optimum conditions showed 5 majors flavonoids.
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Journal: The Journal of Supercritical Fluids - Volume 131, January 2018, Pages 66-71