کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4971002 | 1450306 | 2017 | 5 صفحه PDF | دانلود رایگان |
- Non-destructive Raman spectroscopy technique coupled with super-hydrophobic devices for biological fibers investigation
- Super-hydrophobic surfaces comprising micro-pillars for DNA localization
- Discrimination between A- and B-form of dsDNA suspended between micro-pillars by means of Raman analysis.
- Electron Microscopy preliminary investigation of suspended dsDNA molecules
The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.
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Journal: Microelectronic Engineering - Volume 175, 5 May 2017, Pages 38-42