کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4996676 1368272 2018 8 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Genome hunting of carbonyl reductases from Candida glabrata for efficient preparation of chiral secondary alcohols
ترجمه فارسی عنوان
شکار ژنوم کربونیل ردوکتاز از Candida glabrata برای آماده سازی کارآمد از کربن آلاینده های ثانویه
کلمات کلیدی
Candida glabrata؛ شکار ژنوم؛ Reductase؛ الکل ثانویه کریل؛ شاخص شانن-وینر؛
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


- Cg26 was newly identified from Candida glabrata by genome hunting.
- Average activity and Shannon-Wiener index were evaluated in screening of reductases.
- Cg26 is metal ions dependent.
- (R)-HPBE was enantioselectively synthesized with substrate to biocatalyst ratio of 15.

In this work, genome hunting strategy was adopted in screening for reductases from Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli BL21(DE3). A substrate library containing 32 substrates was established for characterization of each reductase by average specific activity and Shannon-Wiener index. Among them, Cg26 was identified with the highest efficiency and wider substrate spectrum in the reduction of prochiral ketones, with average activity and Shannon-Wiener index of 8.95 U·mg−1 and 2.82. Cg26 is a member of 'extended' short chain dehydrogenase/reductase superfamily. Ni2+ could improve its activity. As much as 150 g·L−1 ethyl 2-oxo-4-phenylbutyrate could be completely converted by 10 g·L−1 Cg26. This study provides evidence for this newly identified Cg26 in the preparation of chiral secondary alcohols.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 247, January 2018, Pages 553-560
نویسندگان
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