کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4996686 1368272 2018 7 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Development of a thermo-stable and recyclable magnetic nanobiocatalyst for bioprocessing of fruit processing residues and D-allulose synthesis
ترجمه فارسی عنوان
توسعه نانوبيوکاتاليست مغناطيسي مقاوم در برابر حرارت و قابل بازيافت براي پردازش بيولوژيک از بقاياي پردازش ميوه و سنتز D-آللوئز
کلمات کلیدی
D-Psicose 3-epimerase؛ بی حرکت سازی آنزیم؛ نانوذرات مغناطیسی؛ پایداری حرارتی؛ بازیافت؛ تفاله میوه؛
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


- Smt3-D-psicose 3-epimerase was immobilized onto functionalized magnetic nanoparticles.
- Immobilization remarkably improved the thermal stability of enzyme.
- The immobilized enzyme showed reusability potential for multiple duty-cycles.
- The nanobiocatalyst was employed for bioprocessing of fruit processing residues and biosynthesis of D-allulose production.

The aim of the study was to covalently immobilize Smt3-D-psicose 3-epimerase onto functionalized iron oxide magnetic nanoparticles. After immobilization, Km of the immobilized enzyme increased, however, Vmax was nearly the same as that of its free form, indicating that immobilization has no detrimental effects on its catalytic output. The covalent immobilization caused a reduction in the deactivation rate constant (kd) values leading to 4-5 fold enhancement in its half-life at 50-65 °C, indicating significant thermal stability of the iron-enzyme nanobioconjugate. The immobilized enzyme showed excellent storage stability by losing only 20% activity even after 60 days of storage at 4 °C. The immobilized enzyme retained up to 90% of its initial activity even after 10 consecutive cycles of catalyzing D-fructose epimerization reactions. Thus, after immobilization the enzyme exhibited remarkable improvements in thermal tolerance, storage stability and recycling efficiency, useful for development of industrially exploitable process for D-allulose production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 247, January 2018, Pages 633-639
نویسندگان
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