کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4996712 1368272 2018 6 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Production of 1,3-propanediol using a novel 1,3-propanediol dehydrogenase from isolated Clostridium butyricum and co-biotransformation of whole cells
ترجمه فارسی عنوان
تولید 1،3-پروپان دیول با استفاده از جدید 1،3-پروپانیدیولدیدروژناز از Clostridium butyricum جدا شده و با همکاری بیوت ترانسفرم شدن کل سلولها
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کلمات کلیدی
butyricum کلستریدیوم؛ گلیسرول؛ 1،3-پروپانیدیول دهیدروژناز؛ انتقال بیوتکنولوژی؛ سلول کامل؛
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
چکیده انگلیسی


- A newly dhaT gene from isolated C. butyricum YJH-09 was cloned and expressed.
- The mixed whole cells was firstly used for producing 1,3-PD based on a novel characterized PDOR.
- The production of 1,3-PD was improved more than 2 times via co-biotransformation.

In this study, a newly strain named Clostridium butyricum YJH-09 were isolated from the sample of pond soil and identified through physiological, biochemical and 16S rDNA analysis. Then, the dhaT gene encoding a novel 1,3-propanediol dehydrogenase (PDOR) was cloned from this strain and expressed in Escherichia coli BL21(DE3). Subsequently, the recombinant PDOR was purified and the optimal pH and temperature, specific activities and kinetic parameter were investigated. Furthermore, the whole cells of Clostridium butyricum YJH-09 mixed with BL21-dhaT were used to produce 1,3-PD through co-biotransformation. As results, 25.88 g/L of 1,3-PD was generated with 0.54 g/g yield from 50 g/L glycerol in 30 h, and the 1,3-PD production was increased more than 2-fold compared with wild type strain alone. This research would offer useful information for further development of the biosynthesis of 1,3-PD.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 247, January 2018, Pages 838-843
نویسندگان
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