|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|4996712||1368272||2018||6 صفحه PDF||سفارش دهید||دانلود کنید|
- A newly dhaT gene from isolated C. butyricum YJH-09 was cloned and expressed.
- The mixed whole cells was firstly used for producing 1,3-PD based on a novel characterized PDOR.
- The production of 1,3-PD was improved more than 2 times via co-biotransformation.
In this study, a newly strain named Clostridium butyricum YJH-09 were isolated from the sample of pond soil and identified through physiological, biochemical and 16S rDNA analysis. Then, the dhaT gene encoding a novel 1,3-propanediol dehydrogenase (PDOR) was cloned from this strain and expressed in Escherichia coli BL21(DE3). Subsequently, the recombinant PDOR was purified and the optimal pH and temperature, specific activities and kinetic parameter were investigated. Furthermore, the whole cells of Clostridium butyricum YJH-09 mixed with BL21-dhaT were used to produce 1,3-PD through co-biotransformation. As results, 25.88Â g/L of 1,3-PD was generated with 0.54Â g/g yield from 50Â g/L glycerol in 30Â h, and the 1,3-PD production was increased more than 2-fold compared with wild type strain alone. This research would offer useful information for further development of the biosynthesis of 1,3-PD.
Journal: Bioresource Technology - Volume 247, January 2018, Pages 838-843