Study on optimization of process parameters for enhancing the multi-hydrolytic enzyme activity in garbage enzyme produced from preconsumer organic waste

- A crude mixed hydrolytic enzyme seems to be a good substitute than single enzyme.
- Garbage enzyme (GE) possesses protease, lipase and amylase activity.
- To produce GE with higher hydrolytic enzyme activity needs optimized parameters.
- Statistical model for GE production were determined.
- GE significantly utilizes for different sustainable environmental applications.

The garbage enzymes produced from preconsumer organic waste containing multi hydrolytic enzyme activity which helps to solubilize the waste activated sludge. The continuous production of garbage enzyme and its scaling up process need a globe optimized condition. In present study the effect of fruit peel composition and sonication time on enzyme activity were investigated. Garbage enzyme produced from 6 g pineapple peels: 4 g citrus peels pre-treated with ultrasound for 20 min shows higher hydrolytic enzymes activity. Simultaneously statistical optimization tools were used to model garbage enzyme production with higher activity of amylase, lipase and protease. The maximum activity of amylase, lipase and protease were predicted to be 56.409, 44.039, 74.990 U/ml respectively at optimal conditions (pH (6), temperature (37 °C), agitation (218 RPM) and fermentation duration (3 days)). These optimized conditions can be successfully used for large scale production of garbage enzyme with higher hydrolytic enzyme activity.

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