کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5009014 1462038 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A highly sensitive aptamer-immunoassay for vascular endothelial growth factor coupled with portable glucose meter and hybridization chain reaction
ترجمه فارسی عنوان
آزمایش ایمونویته آپتامر بسیار حساس برای فاکتور رشد اندوتلیال عروقی همراه با اندازه گیرنده قابل حمل و واکنش زنجیره ای هیبریدیزاسیون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this paper, coupled with hybridization chain reaction and portable personal glucose meter, a highly sensitive aptamer-based immunoassay for vascular endothelial growth factor (VEGF) is fabricated. The antibody immobilized on the microplate captured the target protein, which would be sandwiched by the formation of aptamer/VEGF/antibody complex in the presence of aptamer. And then, an 18-nt elongated strand at the 3′ end of aptamer, with the assist of invertase-functionalized auxiliary probe, triggered the hybridization chain reaction to generate invertase-concatamers. Vast invertase catalyzed sucrose to glucose, which is monitored through personal glucose meter. Finally, this portable sensing system can achieve the detection of limit of 1.2 pg/mL with good stability and selectivity. Meanwhile, VEGF in the serum would also be assayed with satisfied result.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 253, December 2017, Pages 660-665
نویسندگان
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