کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5009108 1462039 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence enhancement thermoresponsive polymer luminescent sensors based on BODIPY for intracellular temperature
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fluorescence enhancement thermoresponsive polymer luminescent sensors based on BODIPY for intracellular temperature
چکیده انگلیسی


- The water soluble copolymer hydrogel poly(NIPAM-co-BODIPY-AA) as nanothermometer has been synthesized.
- poly(NIPAM-co-BODIPY-AA) shows properties including good temperature-responsive reversibility and biocompatibility.
- The fluorescence intensity increased linearly with increasing physiology temperature (37-42 °C).

Herein, A water soluble, visible excited and simple-structured copolymer hydrogel, poly(NIPAM-co-BODIPY-AA), consisting of N-isopropylacrylamide (NIPAM) and derivative of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) units as thermoresponsive and polarity sensitive fluorescent signalling parts, respectively, has been synthesized. The copolymer exhibits weak fluorescence at temperature low than 35 °C in aqueous solution, whereas the red fluorescence intensity (λem = 605 nm) increases with a 10 folds rise in temperature up to 47 °C with an increase of average lifetime from 0.68 ns up to 2 ns in the thermal transition processes. The fluorescence intensity increased linearly with increasing physiology temperature (37-42 °C). The resultant nanothermometer could feature a high and spontaneous uptaking into the BHK cells. The prepared nanothermometer which is user friendly can provide tools for unveiling intrinsic relationship between the intracellular temperature and could be used in further applications for the fluorescent temperature sensing included parts of physiological temperature range in living cells.

poly(NIPAM-co-BODIPY-AA) exhibits fluorescence intensity (λem = 605 nm) increases in temperature from 35 °C up to 47 °C and the fluorescent sensing in living cells.87

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 252, November 2017, Pages 577-583
نویسندگان
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