Neutravidin biosensor for direct capture of dual-functional biotin-molecular beacon-AuNP probe for sensitive voltammetric detection of microRNA

- miRNA-21 biosensor based on of neutravidin electrode and biotin-MB-AuNP label.
- Signal amplification by silver enhancement technique.
- Highly selective and sensitive (LOD of 4.0 × 10−15 M) for miRNA-21 detection.
- Extremely wide analytic dynamic range for detection of miRNA 10 × 10−15-1 × 10−9 M (5 orders of magnitude).
- Detection of miRNA-21 in spiked serum at clinically relevant concentrations.

We have demonstrated a new approach using a neutravidin-based biosensor combined with a dual-function gold nanoparticle (AuNP) biolabel, for simple and sensitive detection of microRNA-21 (miRNA-21). The selectivity of the biosensor is provided by the intrinsic properties of the dual-functional biotin-MB-AuNP label. The assay procedure is relatively simple, exploiting a one-pot assay concept where the affinity capture of the miRNA-21/dual-functional biotin-MB-AuNP complex, via the strong biotin-neutravidin supramolecular interaction, and simultaneous detection of the captured AuNPs label with stripping voltammetry, is performed in a single step. This electrochemical miRNA biosensor could detect miRNA-21 with limit of detection of 0.1 × 10−12 and a dynamic range from 0.5 × 10−12 to 1.0 × 10−9 M. The performance of the miRNA-21biosensor was further improved after silver deposition onto the AuNPs, delivering an enhanced detection limit of 4.0 × 10−15 M of miRNA-21, and an extremely wide analytic dynamic range from 10 × 10−15 to 1 × 10−9 M (5 orders of magnitude). This exceptionally broad dynamic range demonstrates the advantage of the one-pot assay approach with direct capture of the dual functional biotin-MB-AuNP via the strong biotin-neutravidin supramolecular interaction. Furthermore, we demonstrated the detection of miRNA-21 in spiked serum at clinically relevant concentrations. The miRNA biosensor displayed excellent analytical performance for the detection of miRNA and could provide a powerful and convenient tool for biomedical research and applications in cancer diagnostics.