کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
50110 46780 2016 4 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Insight into enzymatic synthesis of phosphatidylserine in deep eutectic solvents
ترجمه فارسی عنوان
نگاهی به سنتز آنزیمی فسفاتیدیلرسین در حلال های عمیق یوتکتیک
کلمات کلیدی
حلال های عمیق یوتکتیک؛ فسفاتیدیلسرین؛ ترانس فسفاتیدیدیل کردن؛ فسفولیپاز D؛ ثبات عملیاتی
Deep eutectic solvents; Phosphatidylserine; Transphosphatidylation; Phospholipase D; Operational stability
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی کاتالیزور
چکیده انگلیسی


• Enzymatic phosphatidylserine synthesis in deep eutectic solvents was developed.
• > 90% yield of phosphatidylserine could be achieved.
• 81% original activity of enzyme was maintained after being used for 10 batches.

Deep eutectic solvents (DESs) have recently attracted a significant interest in numerous fields including biocatalysis because they are inexpensive, environmentally friendly, non-toxic, biodegradable, and enzyme compatible. Herein, these solvents were successfully used as the reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for the synthesis of phosphatidylserine for the first time. Enzymatic phosphatidylserine synthesis in various DESs was comprehensively investigated, employing phospholipase D. Our results indicated that > 90% yield of phosphatidylserine could be achieved using choline chloride/ethylene glycol as DES. Furthermore, 81% original activity of the enzyme was maintained after being used for 10 batches. This study indicates that DESs act as potential candidates for the eco-friendly solvents in biocatalysis applications.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Catalysis Communications - Volume 82, 5 July 2016, Pages 16–19
نویسندگان
, ,