| کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
|---|---|---|---|---|
| 5030771 | 1470932 | 2018 | 4 صفحه PDF | دانلود رایگان |
لغات کلیدی
1. مقدمه
2. مواد و روش ها
2.1 ساخت بیوسنسور گرافن
2.2 اندازه گیری بیوسنسور گرافن
2.3 عدم تحرک پروتئین
3. نتایج و بحث
4. نتیجه گیری
- Combines commercially available graphene sensors with CDC antibodies.
- A sensitive biosensor system developed to detect Zika virus antigen in serum.
- Specificity demonstrated by null response to Japanese Encephalitis virus antigen.
- Demonstrated LLOD and specificity provide opportunity for early stage detection.
We have developed a cost-effective and portable graphene-enabled biosensor to detect Zika virus with a highly specific immobilized monoclonal antibody. Field Effect Biosensing (FEB) with monoclonal antibodies covalently linked to graphene enables real-time, quantitative detection of native Zika viral (ZIKV) antigens. The percent change in capacitance in response to doses of antigen (ZIKV NS1) coincides with levels of clinical significance with detection of antigen in buffer at concentrations as low as 450Â pM. Potential diagnostic applications were demonstrated by measuring Zika antigen in a simulated human serum. Selectivity was validated using Japanese Encephalitis NS1, a homologous and potentially cross-reactive viral antigen. Further, the graphene platform can simultaneously provide the advanced quantitative data of nonclinical biophysical kinetics tools, making it adaptable to both clinical research and possible diagnostic applications. The speed, sensitivity, and selectivity of this first-of-its-kind graphene-enabled Zika biosensor make it an ideal candidate for development as a medical diagnostic test.
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Journal: Biosensors and Bioelectronics - Volume 100, 15 February 2018, Pages 85-88