کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030817 1470932 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Facile synthesis of MoS2@Cu2O-Pt nanohybrid as enzyme-mimetic label for the detection of the Hepatitis B surface antigen
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Facile synthesis of MoS2@Cu2O-Pt nanohybrid as enzyme-mimetic label for the detection of the Hepatitis B surface antigen
چکیده انگلیسی


- A novel and ultrasensitive sandwich-type immunosensor was developed for the quantitative detection of HBs Ag.
- Porous GO/Au was used as platform to improve the loading capacity of primary antibody.
- Use the synergetic effect present in MoS2@Cu2O-Pt to improve the sensitivity.
- The immunosensor exhibited a wide detection range and excellent sensitivity.

An ultrasensitive sandwich-type electrochemical immunosensor was proposed for quantitative detection of hepatitis B surface antigen, which is a representative biomarker of the Hepatitis B virus. First, the porous graphene oxide/Au composites with good conductive ability were employed to accelerate the electron transfer on the electrode interface. Furthermore, the amino functionalized molybdenum disulfide @ cuprous oxide hybrid with coral morphology was prepared to combine platinum nanoparticles for achieving signal amplification strategy. The resulting nanocomposites (molybdenum disulfide @ cuprous oxide - platinum) demonstrated uniform coral morphology, which effectively improved the specific surface area available for loading the secondary antibody and the number of catalytically active sites, even also increased the electrical conductivity. Based on these advantages, this composite system yielded a superior electrocatalytic current response toward the reduction of hydrogen peroxide. In addition, porous graphene oxide/Au composites were used to modify the glassy carbon electrode, thereby presenting a large surface area and becoming biocompatible, for improving the loading capacity of the primary antibody. Under optimal conditions, we obtained a linear relationship between current signal and hepatitis B surface antigen concentration in the broad range from 0.5 pg/mL to 200 ng/mL, with a detection limit of 0.15 pg/mL (signal-to-noise ratio of 3). These values are promising towards clinical applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 100, 15 February 2018, Pages 512-518
نویسندگان
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