کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031330 1470933 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA origami nanorobot fiber optic genosensor to TMV
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
DNA origami nanorobot fiber optic genosensor to TMV
چکیده انگلیسی


- A 3D DNA origami nanorobot has been immobilized on the glass optical fiber tips.
- The chemiluminescence signals have been collected with a portable transducer.
- Our system can be a candidate for point-of-care diagnostics and in vivo biosensing.

In the quest of greater sensitivity and specificity of diagnostic systems, one continually searches for alternative DNA hybridization methods, enabling greater versatility and where possible field-enabled detection of target analytes. We present, herein, a hybrid molecular self-assembled scaffolded DNA origami entity, intimately immobilized via capture probes linked to aminopropyltriethoxysilane, onto a glass optical fiber end-face transducer, thus producing a novel biosensor. Immobilized DNA nanorobots with a switchable flap can then be actuated by a specific target DNA present in a sample, by exposing a hemin/G-quadruplex DNAzyme, which then catalyzes the generation of chemiluminescence, once the specific fiber probes are immersed in a luminol-based solution. Integrating organic nanorobots to inorganic fiber optics creates a hybrid system that we demonstrate as a proof-of-principle can be utilized in specific DNA sequence detection. This system has potential applications in a wide range of fields, including point-of-care diagnostics or cellular in vivo biosensing when using ultrathin fiber optic probes for research purposes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 209-215
نویسندگان
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