کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031347 1470933 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Profuse color-evolution-based fluorescent test paper sensor for rapid and visual monitoring of endogenous Cu2+ in human urine
ترجمه فارسی عنوان
حسگر کاغذ تست فلورسنت متشکل از رنگ انحصاری برای نظارت سریع و بصری از Cu2 + در ادرار انسان
کلمات کلیدی
کاغذ تست فلورسنت؛ سنسور؛ پروب تریکولر؛ تشخیص ویژوال؛ Cu2 +
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- A tricolor probe system was fabricated by simply mixing bCDs, gQDs and rQDs according to volume ratio of 3:9:4.
- Tricolor probe-based principle is that the fluorescence of gQDs and rQDs is quenched by Cu2+ while that of bCDs keeps constant.
- Tricolor probe displays more profuse and wider color variations with increase of Cu2+ amount, compared to dual-color probe.
- The proposed colorimetric method shows high selectivity and sensitivity to Cu2+.
- Fluorescent test paper provides a novel tool for Cu2+ assay and a rapid diagnosis whether we fall in WD or Menkes disease.

The fluorescent paper for colorimetric detection of metal ions has been widely fabricated using various sensing probes, but it still remains an elusive task to design a test paper with multicolor variation with target dosages for accurate determination. Herein, we report a profuse color-evolution-based fluorescent test paper sensor for rapid and visual monitoring of Cu2+ in human urine by printing tricolor probe onto filter paper. The tricolor probe consists of blue-emission carbon dots (bCDs), green-emission quantum dots (gQDs) and red-emission quantum dots (rQDs), which is based on the principle that the fluorescence of gQDs and rQDs are simultaneously quenched by Cu2+, whereas the bCDs as the photostable internal standard is insensitive to Cu2+. Upon the addition of different amounts of Cu2+, the ratiometric fluorescence intensity of the tricolor probe continuously varied, leading to color changes from shallow pink to blue with a detection limit of 1.3 nM. When the tricolor probe solution was printed onto a sheet of filter paper, as-obtained test paper displayed a more profuse color evolution from shallow pink to light salmon to dark orange to olive drab to dark olive green to slate blue to royal blue and to final dark blue with the increase of Cu2+ concentration compared with dual-color probe-based test paper, and dosage scale as low as 6.0 nM was clearly discriminated. The sensing test paper is simple, rapid and inexpensive, and serves as a visual platform for ultrasensitive monitoring of endogenous Cu2+ in human urine.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 332-337
نویسندگان
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