کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031364 1470933 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A portable smart-phone device for rapid and sensitive detection of E. coli O157:H7 in Yoghurt and Egg
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A portable smart-phone device for rapid and sensitive detection of E. coli O157:H7 in Yoghurt and Egg
چکیده انگلیسی


- Smart-phone based fluorescent detector with lower cost was developed for on-site detection of E. coli O157:H7.
- The 3D printed portable biosensor showed better detection accuracy comparable to commercial ELISA.
- Successful conjugation of magnetic beads and FITC achieve rapid and visual detection.
- Sensitive and selective measurements was conducted in yogurt and egg.

The detection of E. coli O157:H7 in foods has held the attention of many researchers because of the seriousness attributed to this pathogen. In this study, we present a simple, sensitive, rapid and portable smartphone based fluorescence device for E. coli O157:H7 detection. This field-portable fluorescent imager on the smartphone involves a compact laser-diode-based photosource, a long-pass (LP) thin-film interference filter and a high-quality insert lenses. The design of the device provided a low noise to background imaging system. Based on a sandwich ELISA and the specific recognition of antibody to E. coli O157:H7, the sensitive detection of E. coli O157:H7 were realized both in standard samples and real matrix in yoghurt and egg on our device. The detection limit are 1 CFU/mL and 10 CFU/mL correspondingly. Recovery percentages of spiked yogurt and egg samples with 103, 104 and 105 CFU/mL E. coli O157:H7 were 106.98, 96.52 and 102.65 (in yogurt) and 107.37, 105.64 and 93.84 (in egg) samples using our device, respectively. Most importantly, the entire process could be quickly completed within two hours. This smartphone based device provides a simple, rapid, sensitive detection platform for fluorescent imaging which applied in pathogen detection for food safety monitoring.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 479-485
نویسندگان
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