کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031365 1470933 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel peptidylated surfaces for interference-free electrochemical detection of cardiac troponin I
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Novel peptidylated surfaces for interference-free electrochemical detection of cardiac troponin I
چکیده انگلیسی


- Self-assembling of peptide dendrons on gold to develop electrochemical sensors.
- Peptidylated surfaces efficiently display capture antibodies increasing the sensitivity.
- Non-triazolic peptide-based sensors gave rise to anti-fouling properties.
- Non-triazolic peptide-based sensors detected troponin I at pg/mL levels.

Novel peptidylated surfaces were designed to minimise interferences when electrochemically detecting cardiac troponin I in complex biological samples. Disulfide-cored peptide dendrons featuring carbomethoxy groups were self-assembled on gold electrodes. The carbomethoxy groups were deprotected to obtain carboxylic groups used to immobilise antibodies for cardiac troponin I marker. The chemisorption of two types of peptides, one containing triazole and the other with native peptide bonds, on a gold substrate was studied by quartz crystal microbalance (QCM), surface plasmon resonance (SPR) and X-ray photoelectron spectroscopy (XPS). Peptides formed ordered self-assembled monolayers, contributing to a more efficient display of the subsequently immobilised antibodies towards their binding to the antigen. As a result, electrochemical immunosensors prepared by self-assembly of peptides afforded higher sensitivities for cardiac troponin I than those prepared by the chemisorption of alkane thiolated compounds. Triazolic peptide-modified immunosensors showed extraordinary sensitivity towards cardiac troponin I [1.7 µA/(ng/mL) in phosphate buffer], but suffered from surface fouling in 10% serum. Modification with non-triazolic peptides gave rise to anti-fouling properties and still enabled the detection of cardiac troponin I at pg/mL concentrations in 10% serum without significant matrix effects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 486-492
نویسندگان
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