Short CommunicationHuman brain endothelial barrier cells are distinctly less vulnerable to silver nanoparticles toxicity than human blood vessel cells: A cell-specific mechanism of the brain barrier?

The blood-brain barrier (BBB) constitutes a distinctive and tightly regulated interface between the brain and the peripheral circulation. The objective of studies was to compare responses of human endothelial cells representing the model of blood vessels - EA.hy926 and HUVEC cells and the model of the brain endothelial barrier - HBEC5i cells to silver nanoparticles (SNPs). A contact of SNPs with endothelial cells resulted in a formation of SNP agglomerates. Consequently, the SNPs uptake by endothelial cells affected cell viability and membrane integrity however observed responses were different. Brain endothelial barrier HBEC5i cells were much less vulnerable to SNPs toxicity comparing to EA.hy926 and HUVEC cells. It can be ascribed to the presence of specialized cellular components of the brain barrier, protecting HBEC5i cells against toxic SNPs. Fundamental understanding of SNPs inducing the BBB dysfunction may initiate engineering novel SNPs which are safe for the BBB and thereby safe for the brain.

Graphical AbstractThis work evaluated the influence of silver nanoparticles (SNPs) on human endothelial cells (HUVEC and EA.hy926) reflecting the functions of human blood vessels and the human cerebral microvascular endothelial cells (HBEC5i) which serve as the brain endothelial barrier model. In vitro studies showed clearly that HBEC5i cells were less susceptible to SNPs toxicity comparing with HUVEC and EA.hy926 cells. It may be attributed to distinct functional and morphological features of the brain microvascular cells.94