کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
504806 864435 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Automated detection and analysis of depolarization events in human cardiomyocytes using MaDEC
ترجمه فارسی عنوان
تشخیص خودکار و تحلیل رویدادهای دپلاریزاسیون در قلب انسان با استفاده از MaDEC
کلمات کلیدی
تصویربرداری رنگ حساس به ولتاژ ؛ کاردیومیوسیت مشتق شده HIPS ؛ تشخیص سیگنال؛ همسان فیلتر؛ تقسیم بندی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی کامپیوتر نرم افزارهای علوم کامپیوتر
چکیده انگلیسی


• We cultured human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs).
• We used voltage-sensitive dyes (VSDs) to image actively beating hiPS-CMs.
• We built a software (MaDEC) to detect and quantify depolarization events of hiPS-CMs.
• MaDEC performed well in both high- and low-SNR scenarios across 3 drug treatments MaDEC is written in MATLAB and freely available.

Optical imaging-based methods for assessing the membrane electrophysiology of in vitro human cardiac cells allow for non-invasive temporal assessment of the effect of drugs and other stimuli. Automated methods for detecting and analyzing the depolarization events (DEs) in image-based data allow quantitative assessment of these different treatments. In this study, we use 2-photon microscopy of fluorescent voltage-sensitive dyes (VSDs) to capture the membrane voltage of actively beating human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs). We built a custom and freely available Matlab software, called MaDEC, to detect, quantify, and compare DEs of hiPS-CMs treated with the β-adrenergic drugs, propranolol and isoproterenol. The efficacy of our software is quantified by comparing detection results against manual DE detection by expert analysts, and comparing DE analysis results to known drug-induced electrophysiological effects. The software accurately detected DEs with true positive rates of 98–100% and false positive rates of 1–2%, at signal-to-noise ratios (SNRs) of 5 and above. The MaDEC software was also able to distinguish control DEs from drug-treated DEs both immediately as well as 10 min after drug administration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Computers in Biology and Medicine - Volume 75, 1 August 2016, Pages 109–117
نویسندگان
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