کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5130738 | 1490853 | 2017 | 7 صفحه PDF | دانلود رایگان |
- Meso/macroporous cobaltous oxide nanoflakes were electrodeposited.
- The nanoflakes were employed to fabricate an influenza biosensor.
- The biosensor was applied to detect clinical samples.
Meso/macroporous cobalt (II) oxide nanoflakes were electrodeposited in a one-step process in the presence of N-methylpyrrolidone. On the surface of nanoflakes, a specific single stranded DNA sequence from the genome of influenza A subtype was then immobilized to fabricate an electrochemical biosensor. Hybridization of the biosensor with complementary, non-complementary and base-mismatch sequences was electrochemically detected. The biosensor was also employed to detect complementary DNA of viral RNA in culture and human samples. The biosensor could detect the complementary sequence with a detection limit of 86.4 amol Lâ1 and a linear concentration range of 1.0 fmol Lâ1 to 1.0 nmol Lâ1. It also detected a complementary DNA sequence converted from viral RNA with a detection limit of 0.28 ng μLâ1 in a linear concentration range of 0.5-10 ng μLâ1. Low detection limit, simple method of preparation of the transducer and no needing any DNA strand modification and tag are the principal advantages of the biosensor.
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Journal: Analytica Chimica Acta - Volume 979, 1 August 2017, Pages 51-57