|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5131439||1491308||2017||3 صفحه PDF||سفارش دهید||دانلود کنید|
A variety of techniques were applied to wool follicles stored in William's E culture medium to optimise the extraction of keratin and keratin associated proteins (KAPs). A time course study indicated that the maximum storage time for live skin in this buffer at 20Â Â°C was 24Â h, after which degradative loss of protein became significant. Maceration of the skin for 10Â min followed by reciprocal action shaking for 14Â h had a detrimental effect on keratin extractability. The best approach involved using a Dounce homogeniser as this resulted in the highest amount of Type I and II keratins and KAPs.
Journal: Analytical Biochemistry - Volume 539, 15 December 2017, Pages 8-10